Structural insights into immune recognition of the severe acute respiratory syndrome coronavirus S protein receptor binding domain.
Identifieur interne : 002A09 ( Main/Exploration ); précédent : 002A08; suivant : 002A10Structural insights into immune recognition of the severe acute respiratory syndrome coronavirus S protein receptor binding domain.
Auteurs : John E. Pak [Canada] ; Chetna Sharon ; Malathy Satkunarajah ; Thierry C. Auperin ; Cheryl M. Cameron ; David J. Kelvin ; Jayaraman Seetharaman ; Alan Cochrane ; Francis A. Plummer ; Jody D. Berry ; James M. RiniSource :
- Journal of molecular biology [ 1089-8638 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux (), Anticorps monoclonaux (immunologie), Cristallographie aux rayons X, Données de séquences moléculaires, Fragments Fab d'immunoglobuline (), Fragments Fab d'immunoglobuline (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Lignée cellulaire, Modèles moléculaires, Peptidyl-Dipeptidase A (), Peptidyl-Dipeptidase A (immunologie), Production d'anticorps (immunologie), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (métabolisme), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Sites de fixation, Souris, Structure tertiaire des protéines, Virus du SRAS (immunologie).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de fusion recombinantes, Protéines de l'enveloppe virale.
- immunologie : Anticorps monoclonaux, Fragments Fab d'immunoglobuline, Glycoprotéines membranaires, Peptidyl-Dipeptidase A, Production d'anticorps, Protéines de l'enveloppe virale, Virus du SRAS.
- métabolisme : Protéines de fusion recombinantes.
- Animaux, Anticorps monoclonaux, Cristallographie aux rayons X, Données de séquences moléculaires, Fragments Fab d'immunoglobuline, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Lignée cellulaire, Modèles moléculaires, Peptidyl-Dipeptidase A, Protéines de l'enveloppe virale, Sites de fixation, Souris, Structure tertiaire des protéines.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (chemistry), Antibodies, Monoclonal (immunology), Antibody Formation (immunology), Binding Sites, Cell Line, Crystallography, X-Ray, Humans, Immunoglobulin Fab Fragments (chemistry), Immunoglobulin Fab Fragments (immunology), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Models, Molecular, Molecular Sequence Data, Peptidyl-Dipeptidase A (chemistry), Peptidyl-Dipeptidase A (immunology), Protein Structure, Tertiary, Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (metabolism), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , chemistry : Antibodies, Monoclonal, Immunoglobulin Fab Fragments, Membrane Glycoproteins, Peptidyl-Dipeptidase A, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Recombinant Fusion Proteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal, Immunoglobulin Fab Fragments, Membrane Glycoproteins, Peptidyl-Dipeptidase A, Viral Envelope Proteins.
- immunology : Antibody Formation, SARS Virus.
- chemical , metabolism : Recombinant Fusion Proteins.
- Animals, Binding Sites, Cell Line, Crystallography, X-Ray, Humans, Mice, Models, Molecular, Molecular Sequence Data, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus.
Abstract
The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for host cell attachment and fusion of the viral and host cell membranes. Within S the receptor binding domain (RBD) mediates the interaction with angiotensin-converting enzyme 2 (ACE2), the SARS-CoV host cell receptor. Both S and the RBD are highly immunogenic and both have been found to elicit neutralizing antibodies. Reported here is the X-ray crystal structure of the RBD in complex with the Fab of a neutralizing mouse monoclonal antibody, F26G19, elicited by immunization with chemically inactivated SARS-CoV. The RBD-F26G19 Fab complex represents the first example of the structural characterization of an antibody elicited by an immune response to SARS-CoV or any fragment of it. The structure reveals that the RBD surface recognized by F26G19 overlaps significantly with the surface recognized by ACE2 and, as such, suggests that F26G19 likely neutralizes SARS-CoV by blocking the virus-host cell interaction.
DOI: 10.1016/j.jmb.2009.03.042
PubMed: 19324051
Affiliations:
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Le document en format XML
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Pak</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Molecular Genetics, University of Toronto, Toronto, Ontario</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name></author><author><name sortKey="Satkunarajah, Malathy" sort="Satkunarajah, Malathy" uniqKey="Satkunarajah M" first="Malathy" last="Satkunarajah">Malathy Satkunarajah</name></author><author><name sortKey="Auperin, Thierry C" sort="Auperin, Thierry C" uniqKey="Auperin T" first="Thierry C" last="Auperin">Thierry C. Auperin</name></author><author><name sortKey="Cameron, Cheryl M" sort="Cameron, Cheryl M" uniqKey="Cameron C" first="Cheryl M" last="Cameron">Cheryl M. Cameron</name></author><author><name sortKey="Kelvin, David J" sort="Kelvin, David J" uniqKey="Kelvin D" first="David J" last="Kelvin">David J. Kelvin</name></author><author><name sortKey="Seetharaman, Jayaraman" sort="Seetharaman, Jayaraman" uniqKey="Seetharaman J" first="Jayaraman" last="Seetharaman">Jayaraman Seetharaman</name></author><author><name sortKey="Cochrane, Alan" sort="Cochrane, Alan" uniqKey="Cochrane A" first="Alan" last="Cochrane">Alan Cochrane</name></author><author><name sortKey="Plummer, Francis A" sort="Plummer, Francis A" uniqKey="Plummer F" first="Francis A" last="Plummer">Francis A. Plummer</name></author><author><name sortKey="Berry, Jody D" sort="Berry, Jody D" uniqKey="Berry J" first="Jody D" last="Berry">Jody D. Berry</name></author><author><name sortKey="Rini, James M" sort="Rini, James M" uniqKey="Rini J" first="James M" last="Rini">James M. Rini</name></author></analytic><series><title level="j">Journal of molecular biology</title><idno type="eISSN">1089-8638</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (chemistry)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibody Formation (immunology)</term><term>Binding Sites</term><term>Cell Line</term><term>Crystallography, X-Ray</term><term>Humans</term><term>Immunoglobulin Fab Fragments (chemistry)</term><term>Immunoglobulin Fab Fragments (immunology)</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Peptidyl-Dipeptidase A (chemistry)</term><term>Peptidyl-Dipeptidase A (immunology)</term><term>Protein Structure, Tertiary</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (metabolism)</term><term>SARS Virus (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux ()</term><term>Anticorps monoclonaux (immunologie)</term><term>Cristallographie aux rayons X</term><term>Données de séquences moléculaires</term><term>Fragments Fab d'immunoglobuline ()</term><term>Fragments Fab d'immunoglobuline (immunologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Lignée cellulaire</term><term>Modèles moléculaires</term><term>Peptidyl-Dipeptidase A ()</term><term>Peptidyl-Dipeptidase A (immunologie)</term><term>Production d'anticorps (immunologie)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Sites de fixation</term><term>Souris</term><term>Structure tertiaire des protéines</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Immunoglobulin Fab Fragments</term><term>Membrane Glycoproteins</term><term>Peptidyl-Dipeptidase A</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Recombinant Fusion Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Immunoglobulin Fab Fragments</term><term>Membrane Glycoproteins</term><term>Peptidyl-Dipeptidase A</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de fusion recombinantes</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Fragments Fab d'immunoglobuline</term><term>Glycoprotéines membranaires</term><term>Peptidyl-Dipeptidase A</term><term>Production d'anticorps</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Antibody Formation</term><term>SARS Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de fusion recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Binding Sites</term><term>Cell Line</term><term>Crystallography, X-Ray</term><term>Humans</term><term>Mice</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Protein Structure, Tertiary</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Cristallographie aux rayons X</term><term>Données de séquences moléculaires</term><term>Fragments Fab d'immunoglobuline</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Modèles moléculaires</term><term>Peptidyl-Dipeptidase A</term><term>Protéines de l'enveloppe virale</term><term>Sites de fixation</term><term>Souris</term><term>Structure tertiaire des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for host cell attachment and fusion of the viral and host cell membranes. Within S the receptor binding domain (RBD) mediates the interaction with angiotensin-converting enzyme 2 (ACE2), the SARS-CoV host cell receptor. Both S and the RBD are highly immunogenic and both have been found to elicit neutralizing antibodies. Reported here is the X-ray crystal structure of the RBD in complex with the Fab of a neutralizing mouse monoclonal antibody, F26G19, elicited by immunization with chemically inactivated SARS-CoV. The RBD-F26G19 Fab complex represents the first example of the structural characterization of an antibody elicited by an immune response to SARS-CoV or any fragment of it. The structure reveals that the RBD surface recognized by F26G19 overlaps significantly with the surface recognized by ACE2 and, as such, suggests that F26G19 likely neutralizes SARS-CoV by blocking the virus-host cell interaction.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><noCountry><name sortKey="Auperin, Thierry C" sort="Auperin, Thierry C" uniqKey="Auperin T" first="Thierry C" last="Auperin">Thierry C. Auperin</name><name sortKey="Berry, Jody D" sort="Berry, Jody D" uniqKey="Berry J" first="Jody D" last="Berry">Jody D. Berry</name><name sortKey="Cameron, Cheryl M" sort="Cameron, Cheryl M" uniqKey="Cameron C" first="Cheryl M" last="Cameron">Cheryl M. Cameron</name><name sortKey="Cochrane, Alan" sort="Cochrane, Alan" uniqKey="Cochrane A" first="Alan" last="Cochrane">Alan Cochrane</name><name sortKey="Kelvin, David J" sort="Kelvin, David J" uniqKey="Kelvin D" first="David J" last="Kelvin">David J. Kelvin</name><name sortKey="Plummer, Francis A" sort="Plummer, Francis A" uniqKey="Plummer F" first="Francis A" last="Plummer">Francis A. Plummer</name><name sortKey="Rini, James M" sort="Rini, James M" uniqKey="Rini J" first="James M" last="Rini">James M. Rini</name><name sortKey="Satkunarajah, Malathy" sort="Satkunarajah, Malathy" uniqKey="Satkunarajah M" first="Malathy" last="Satkunarajah">Malathy Satkunarajah</name><name sortKey="Seetharaman, Jayaraman" sort="Seetharaman, Jayaraman" uniqKey="Seetharaman J" first="Jayaraman" last="Seetharaman">Jayaraman Seetharaman</name><name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name></noCountry><country name="Canada"><region name="Ontario"><name sortKey="Pak, John E" sort="Pak, John E" uniqKey="Pak J" first="John E" last="Pak">John E. Pak</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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